Yaroslavl State University (Yaroslavl, Russia):

Shekhovtsova N. V., Head of a Chair, e-mail: ninval@mail.ru

For the first time, the microbiota structure of rocks, revealed by Vorotilovo deep well, was reconstructed via analysis of lipid biomarkers. Vorotilovo deep well corresponds to impact origin of Puchezh-Katun ring structure.

The hydrocarbon oxidizing, methylotrophic and iron reducing bacteria are singled out along with microorganisms of sulfur cycle. The basis for the functioning of underground microbiota is the oxidation of hydrocarbon gases by alkanotrophic rhodococcus, which metabolites are conductive to adaptation of methylotrophic bacteria Leifsonia and Planomicrobium to the thermal environment. Presence of these bacteria in rocks was found for the first time.
In addition to syntrophic and biostagnant interactions, ability to formation of spores and nanoforms is the adaptation of microorganisms to survival in underground environment. This article shows the discussion of the scientific and practical importance of the carried out researches.

1. Glubokoe burenie v Puchezh-Katunkskoy impaktnoy strukture (Deep hole drilling in Puchezh-Katun impact structure). Saint Petersburg : A. P. Karpinsky Russian Geological Research Institute, 1999, 392 p.
2. Frick M. G., Belokon T. V., Kalachnikova I. G., Ilarionov S. A. Biomarkers from core samples of superdeep boreholes. The 1996 International Symposium on Subsurface Microbiology (ISSM-96). Davos, Switzerland. September, 1996: Abstracts. Davos, 1997, pp. 106.

3. Bakterialnaya paleontologiya (Bacterial paleontology). Under the editorship of A. Yu. Rozanov. Moscow : Paleontology Institute of Russian Academy of Sciences, 2002, 188 p.
4. Ilarionov S. A., Oborin A. A., Seleznev I. A. et al. Mikrobiologicheskoe issledovanie obraztsov kerna Tyumenskoy sverkhglubokoy skvazhiny (Microbiological research of core samples of Tyumen ultradeep well). Tyumenskaya sverkhglubokaya skvazhina (interval 0–7502 m). Rezultaty bureniya i issledovaniya : sbornik dokladov (Tyumen ultradeep well (0–7502 m). Results of drilling and researches: collection of reports). Nauchnoe burenie v Rossii. Vypusk 4 (Scientific drilling in Russia. Issue 4). Perm : Kama Scientific and Research Institute of Complex Researches of Deep and Ultradeep Wells, 1996, 376 p.
5. Shekhovtsova N. V., Osipov G. A., Verkhovtseva N. V., Pevzner L. A. Analysis of lipid biomarkers in rocks of Archean crystalline basement. Proceedings of SPIE, 2003, Vol. 4939, pp. 160–168.
6. Vasilev S. V., Rudyy A. S., Prikhodko O. Yu. et al. Integral — Integral, 2011, No. 3 (59), pp. 10–12.
7. Gold Th. The deep, hot biosphere. Microbiology, 1992, Vol. 89, pp. 6045–6049.
8. The Microbiology of the Terrestrial Deep Subsurface. Under the editorship of P. S. Amy, D. L. Haldeman. New York [etc]: Lewis Publishers, 1997, 356 p.
9. Metody pochvennoy mikrobiologii i biokhimii (Methods of soil microbiology and geochemistry). Under the editorship of D. G. Zvyagintsev. Moscow : Moscow State University, 1991, 304 p.
10. Batrakov S. G., Rodionova T. A., Esipov S. E. et al. A novel lipopeptide, an inhibitor of bacterial adhesion from the thermophilic and halotolerant subsurface Bacillus licheniformis 603. Biochimica et Biophysica Acta (BBA), 2003, Vol. 1634, No. 3, pp. 107–115.